Assay of crystal violet binding for rapid identification of virulent plasmid-bearing clones of Yersinia enterocolitica
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چکیده
منابع مشابه
Simple assay of calcium dependency for virulent plasmid-bearing clones of Yersinia enterocolitica.
A simplified procedure to detect the calcium dependency of virulent plasmid-bearing strains of Yersinia enterocolitica was developed. A low-calcium, agarose-based medium of brain heat infusion with added magnesium effectively differentiated plasmid-bearing and plasmidless isolates. Further, the expression of calcium dependency in plasmid-bearing strains of Y. enterocolitica as measured by the a...
متن کاملSimple method for demonstration of differential colony morphology of plasmid-associated virulent clones of Yersinia enterocolitica.
Virulent (plasmid-associated) strains of Yersinia enterocolitica grown on RPMI 1640 agar (RPMI-1640 medium [Flow Laboratories] with 40 mM HEPES and 1.5% [wt/vol] purified agar [Difco]) dissociated into small and large colonies. The autoagglutination test (a marker of virulence) is regularly positive with small colonies and negative with large colonies. Avirulent Y. enterocolitica strains gave o...
متن کاملDirect detection and isolation of plasmid-bearing virulent serotypes of Yersinia enterocolitica from various foods.
A procedure was developed for direct detection, isolation, and maintenance of plasmid-bearing virulent serotypes of Yersinia enterocolitica from different food sources. Plasmid-bearing virulent strains of Y. enterocolitica representing five serotypes were simultaneously detected and isolated from enriched swab samples of artificially contaminated pork chops, ground pork, cheese, and zucchini, u...
متن کاملEnrichment, isolation, and virulence of freeze-stressed plasmid-bearing virulent strains of Yersinia enterocolitica on pork.
The influence of freeze stress at -20 degrees C on the enrichment, isolation, detection, presence of virulence plasmid, and expression of virulence of plasmid-bearing Yersinia enterocolitica (YEP+) inoculated on pork chop medallions was assessed. Pork chop medallions (10 cm2) artificially contaminated with 10, 1, and 0.5 CFU/cm2 of YEP+ strains (serotype O:3) were placed in sterile petri dishes...
متن کاملUse of a single procedure for selective enrichment, isolation, and identification of plasmid-bearing virulent Yersinia enterocolitica of various serotypes from pork samples.
Many selective enrichment methods for the isolation of Yersinia enterocolitica from foods have been described. However, no single isolation procedure has been described for the recovery and identification of various plasmid-bearing serotypes. A single improved procedure for selective enrichment, isolation, identification, and maintenance of plasmid-bearing virulent serotypes of Y. enterocolitic...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1987
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.25.6.1039-1042.1987